ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
The column dimension is identical. The column is stuffed with silica particles which happen to be modified to create them non-polar. This is completed by attaching lengthy hydrocarbon chains (8–18 C atoms) to its surface area.
전자를 '고정상', 후자를 '이동상'이라 부르며 크로마토그래피에서는 분석자는 고정상과 이동상의 조합에 의해 분석물의 분리를 제어할 수 있게 됩니다.따라서 분석물, 고정상, 이동상, 세 가지 특성의 이해가 크로마트그래피에서 매우 중요합니다.
By subsequent the following pointers and systematically addressing likely brings about, you can effectively troubleshoot typical HPLC complications and ensure your analyses are exact and trusted.
物質にエネルギーを与える（励起）ことにより発光する（蛍光）性質を利用した検出器。一般に選択性が高く高感度で、物質に特異的な検出が可能。蛍光する性質を持たない物質については、その物質を標識することにより検出が可能になる。
분석물의 피크 면적 값(=검출기의 응답)은 정량화를 위해 사용됩니다. 분석자는 분석을 수행하기 전, 분석물의 표준 용액(기지 농도의 시액)을 몇 가지 측정하고, 시료 농도와 획득한 피크 면적 값에 의해 도표된 검량선을 그립니다.
In a gasoline chromatograph the strain from a compressed gasoline cylinder is adequate to press the mobile period throughout the column. Pushing a liquid cellular section by way of a column, nonetheless, normally takes an awesome offer a lot more hard work, making pressures in surplus of various hundred atmospheres.
It achieves this by exploiting get more info the differing interactions of sample compounds with two crucial phases: the cell phase and the stationary section. Knowledge the core parts of the HPLC system as well as their roles is important for productive Examination.
Lots of differing types of detectors have been use to monitor HPLC separations, nearly all here of which make use of the spectroscopic tactics from Chapter ten or maybe the electrochemical procedures from Chapter eleven.
Ion-exchange chromatography is based within the separation of substances primarily based on their cost. The stationary section contains charged teams that attract and retain oppositely charged ions in the sample.
The HPLC column houses the stationary period, a crucial element for separating analytes. Selecting the suitable column is vital:
Numerous differing types of detectors are actually use to monitor HPLC separations, nearly all of which utilize the spectroscopic methods from Chapter 10 or maybe the electrochemical methods from Chapter 11.
are developed by reacting the silica particles having an organochlorosilane of the general kind Si(CH3)2RCl, where R is undoubtedly an alkyl or substituted alkyl team.
In liquid–liquid chromatography the stationary stage is a liquid movie coated on the packing materials, typically three–10 μm porous silica particles. As the stationary stage may be partly soluble inside the cell phase, it may well elute, or bleed through the column as time passes.